Antonia Red-赖氨酸-葡聚糖

货号: A491542
只有 %1 左
别名
A290969;A290973;A290978;A397491;A290981
储存条件
室温,充氩
运输条件
常规运输
价格
低至 ¥531.90

概述

Antononia Red是一种红移染料,最大吸光度为583 nm,发射波长为602 nm。安东尼娅红赖氨酸葡聚糖易于与生物分子和表面结合,并在细胞和组织中有效固定。所有批次均严格控制分子量、外观、溶解度、取代度、荧光和游离染料。阿拉丁实验室生产4kDa至150kDa的安东尼娅红葡聚糖。安东尼娅红赖氨酸葡聚糖作为紫色粉末提供。找不到你要找的东西?我们随时可以为您定制产品。合成和结构安东尼奥红赖氨酸-葡聚糖组分由特征良好的葡聚糖组份合成,葡聚糖组分得自用安东尼奥红标记的肠系膜明串珠菌。取代度介于0.001-0.01之间。赖氨酸葡聚糖由特征良好、产自明串珠杆菌的葡聚糖级分合用赖氨酸标记肠系膜。然后在温和条件下用安东尼亚红官能化赖氨酸葡聚糖形成安东尼奥红赖氨酸葡聚糖的条件。纯化后将产物控制在Mw,外观、溶解度、DS、pH、游离赖氨酸和游离染料。该产品由制造商指定所用葡聚糖级分的近似分子量。因此,例如,产品Antonia红色赖氨酸葡聚糖4的分子量约为4000 Da。实际分子量为:由GPC确定。该值随分析证书一起提供。葡聚糖基本上是线性α-(1-6)-连接的葡萄糖链,然而,沿链分布的α-(1-3)分支的百分比很低(2-5%)。葡聚糖使用的馏分的分子量为4000至500000,吸光度和其他控制参数。物理性质Antonia Red是一种新型红移染料,类似于德克萨斯红,由阿拉丁开发和生产

咨询公司AB.Antonia Red赖氨酸葡聚糖是紫色粉末,易溶于水或水电解质溶液。安东尼娅红赖氨酸葡聚糖不溶于大多数有机溶剂乙醇、甲醇、丙酮、氯仿、乙酸乙酯等。取代度(DS)介于0.01-0.02(摩尔赖氨酸/摩尔葡萄糖)和0.001-0.01(摩尔安东尼奥红/摩尔葡萄糖)。光谱数据:安东尼娅红赖氨酸葡聚糖在pH 9.0的硼酸盐缓冲液中的最大吸光度为583nm,发射波长为602nm尚未对安东尼奥红赖氨酸葡聚糖进行前瞻性稳定性研究。然而葡聚糖和赖氨酸与葡聚糖的尿素键的结构性质链以及赖氨酸和安东尼娅红之间的共价键将表明该化合物的高稳定性产品。建议将产品储存在气密容器中。安东尼娅红赖氨酸葡聚糖可在环境温度下储存。

安东尼娅红赖氨酸葡聚糖为生物分子提供了简单的结合,并可用于将细胞保持在“类似生命”的状态。安东尼娅红显示出明亮的荧光,具有良好的光稳定性和化学稳定性,并且不依赖于pH。携带氨基的葡聚糖作为生物结合和固定在生命系统中的通用工具1对科学界很有价值。对于结合,赖氨酸结构的游离氨基将能够共价结合到活化的实体,如NHS酯或异硫氰酸酯,或使用常见的肽偶联试剂(如DCC)与羧酸偶联,霍伯特和或哈图。进行细胞和组织固定是为了将组分保持在“类似生命的状态”,并使细胞具有渗透性,以允许抗体进入细胞结构,例如用于显微镜研究或免疫染色。赖氨酸葡聚糖上的氨基与固定剂(此处为甲醛或戊二醛)反应,与蛋白质和脂质等生物分子形成共价交联,固定整个系统和葡聚糖。在分子成像中定性或定量评估生物事件时,这一点尤为重要。如果没有固定,生命系统中的这些结构将迅速解体和扩散。安东尼娅红是一种荧光染料,其最大激发波长为583nm,最大发射波长为611nm,与流行的德克萨斯红染料类似。



产品规格与参数:

SKU 名称 分子量
A491542-4kDa Antonia Red-赖氨酸-葡聚糖 4kDa
A491542-20kDa Antonia Red-赖氨酸-葡聚糖 20kDa
A491542-40kDa Antonia Red-赖氨酸-葡聚糖 40kDa
A491542-70kDa Antonia Red-赖氨酸-葡聚糖 70kDa
A491542-150kDa Antonia Red-赖氨酸-葡聚糖 150kDa

mesenteroides labelled with Lysine. Lysine dextran is then functionalized with Antonia Red under mild

conditions to form Antonia Red Lysine Dextran. After purification, the products are controlled for Mw,

appearance, solubility, DS, pH, free Lysine and free dye. The products are designated by the

approximate molecular weights of the dextran fractions used. Thus, for example, the product Antonia

Red Lysine Dextran 4 has a molecular weight of approx. 4000 Da. The actual molecular weight is

determined by GPC. This value is supplied with the Certificate of Analysis. The dextran is essentially a linear α-(1-6)-linked glucose chain with

however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran

fractions used are from Mw of 4000 to 500000 and are carefully controlled by GPC, optical rotation,

absorbance and other control parameters.Physical propertiesAntonia Red is a novel red-shifted dye similar to Texas Red and is developed and produced by Aladdin

Consultancy AB. Antonia Red Lysine Dextrans are purple powders that are readily soluble in water or

electrolyte solutions. Antonia Red Lysine Dextran are insoluble in most organic solvents, such as

ethanol, methanol, acetone, chloroform, ethyl acetate etc. The degree of substitution, (DS) is between

0.01-0.02 (mol Lysine/mol Glucose) and 0.001-0.01 (mol Antonia Red/mol Glucose).Spectral data Antonia Red-lysine-dextran has an absorbance maximum at 583nm and an emission wavelength of 602 nm in borate buffer at pH 9.0.Stability

No prospective stability studies on Antonia Red Lysine Dextrans have been performed yet. However,

the structural properties of the dextran and of the carbamide linkage of the Lysine to the dextran

chain as well as the convalent bond between Lysine and Antonia Red would suggest high stability of

the product. It is recommended that the products are stored in air-tight containers. Antonia Red

Lysine Dextrans may be stored at ambient temperatures. Application

Antonia Red-lysine-dextrans offer easy conjugation for biomolecules and may be used for preserving cells in a” life like” state. Antonia Red shows bright fluorescence, has good photo- and chemical stability and is non-pH dependent.Dextrans carrying amino groups are valuable to the scientific community as versatile tools for bioconjugation and fixation in living systems1. For conjugation, the free amino group of the Lysine structure will be able to covalently bind to activated entities such as NHS-esters or isothiocyanates or be coupled to carboxylic acids using common peptide coupling reagents such as DCC, HOBt and or HATU. Cell and tissue fixation is performed in order to preserve components in a “life-like state” and to make cells permeable to allow antibodies to access cellular structures, for example for microscopy studies or immunostaining. The amino groups on Lysine Dextran reacts with the fixing agent (here: formaldehyde or glutaraldehyde) to form a covalent crosslinking with biomolecules such as proteins and lipids, immobilizing the whole system and the dextran. This is of particular importance when evaluating biological events qualitatively or quantitatively in molecular imaging. Without fixation, these structures within a living system would fall apart and diffuse rapidly. Antonia Red is a fluorescent dye that has an excitation maximum at 583 nm and an emission maximum at 611 nm, making it an analogue of the popular Texas Red dye.



Product specifications and parameters:

SKU NAME Molecular Weight
A491542-4kDa Antonia Red-lysine-dextran 4kDa
A491542-20kDa Antonia Red-lysine-dextran 20kDa
A491542-40kDa Antonia Red-lysine-dextran 40kDa
A491542-70kDa Antonia Red-lysine-dextran 70kDa
A491542-150kDa Antonia Red-lysine-dextran 150kDa

规格

别名
A290969;A290973;A290978;A397491;A290981
英文别名
Antonia Red™-lysine-dextran
英文名称
Antonia Red-lysine-dextran
储存条件
室温,充氩
运输条件
常规运输
名称和识别符
分子类型
聚合物

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高级数据

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溶解性
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对湿度敏感
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批号(Lot Number) 证书类型 货号
C2619101 分析证书 A491542
F2310765 分析证书 A491542
F2310766 分析证书 A491542
E2317397 分析证书 A491542
E2317399 分析证书 A491542
E2317398 分析证书 A491542
E2317402 分析证书 A491542
E2317394 分析证书 A491542
E2317388 分析证书 A491542
E2317391 分析证书 A491542
K2520056 分析证书 A491542
E2317390 分析证书 A491542
E2527021 分析证书 A491542
E2317387 分析证书 A491542
E2317395 分析证书 A491542
F2611211 分析证书 A491542
E2317401 分析证书 A491542
G2502029 分析证书 A491542
E2317405 分析证书 A491542
G2501013 分析证书 A491542
E2317393 分析证书 A491542
E2317384 分析证书 A491542
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