E2329399计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Antibody Enhancer is a signal enhancing buffer that can be used for the dilution, stabilization and binding capacity enhancement of concentrated antibodies and conjugated antibodies. The product secures molecular conformation and prevents loss of activity, of the diluent protein, over time.
Antibody Enhancer enables users to create prediluted ready-to-use conjugated antibodies (free poly- and monoclonal antibodies, HRP-conjugated antibodies, AP-conjugated antibodies and fluorescence-conjugated antibodies):
Reduces background and provides highest signal-to-noise ratio due to the antibody’s enhanced binding capacity.
Amplifies 40-80% the specific antibody’s activity without increasing the non-specific binding.
Allows for the diluted conjugate to be stored at least 4 years at 2-8 °C.
Minimizes assay errors related to daily dilution variation.
Limited or expensive conjugates can be further diluted without losing sensitivity.
Designed for use in colorimetric as well as in chemiluminescence based assays such as ELISA, Western Blotting and IHC.
Product Characteristics
Antibody Enhancer is a signal enhancing buffer that can be used for the dilution, stabilization and binding capacity enhancement of concentrated antibodies and conjugated antibodies. The product secures molecular conformation and prevents loss of activity of the diluent protein, over time. Antibody Enhancer enables users to create prediluted ready-to-use conjugated antibodies (free poly- and monoclonal antibodies, HRP-conjugated antibodies, AP-conjugated antibodies and fluorescence-conjugated antibodies):
• Reduces background and provides highest signal-to-noise ratio due to the antibody’s enhanced binding capacity.
• Amplifies 40-80% the specific antibody’s activity without increasing the non-specific binding.
• Allows for the diluted conjugate to be stored at least 4 years at 2-8 °C.
• Minimizes assay errors related to daily dilution variation.
• Limited or expensive conjugates can be further diluted without losing sensitivity.
• Designed for use in colorimetric as well as in chemiluminescence based assays such as ELISA, Western Blotting and IHC.
Composition & Properties
The buffer is ready-to-use and BSA free. It contains no azide, mercury or other toxic preservative formulations.
Working Procedure
1.Make a series of dilutions of your antibody/antibody conjugate in the Antibody Enhancer in order to determine the optimal dilution factor (the enhancement is significant in dilutions higher than 1:2000, compared to HRP-StabilPLUS (cat. no. H494387)).
2.Run the assay as usual or store the diluted, conjugated protein preferably at 2-8 °C.
Tips & Tricks
• Antibody Enhancer cannot be used for the dilution of antibodies directed against rabbit immunoglobulins i.e. Swine anti-Rabbit Ig & Goat anti-Rabbit Ig etc. UNI-StabilPLUS (cat. no. U494463) is recommended for this.
Handling & Storage
• Store solution at 2-8 °C.
规格
别名
抗体强化剂
规格或纯度
Ready-to-use (RTU)
英文名称
Antibody Enhancer
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
图片
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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