计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product introduction：
Hydrazide-PEG4-Desthiobiotin is a pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification.
Features of EZ-Link Hydrazide-PEG4-Desthiobiotin:
• Desthiobiotin—analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Glycoprotein labeling—tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads
• Cell surface labeling—tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
• Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Pegylated—spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
This desthiobiotin variant of biotin is activated to label periodate-oxidized carbohydrates of macromolecules. The hydrazide group conjugates to carbonyls (aldehydes and ketones), such as those created from sialic acid and other sugar components of glycoprotein polysaccharides upon treatment with 1 to 10 mM sodium periodate (NaIO4). The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative topolyethylene glycol (PEG) spacer arm enhances solubility and reduces aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules. The No-Weigh Format (5 x 1 mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from degradation.
Desthiobiotin vs. Biotin
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (1/Kd = 10^11 vs. 10^15M, respectively)[1-2]. Consequently, desthiobiotinylated bait proteins and their interacting partners can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down assay experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. The modified avidin-biotin affinity system also eliminates the need to use harsh elution conditions that might disassociate complexes and/or damage the target protein or cell. Desthiobiotin-based techniques are ideal when using native or recombinant proteins that are not expressed with a fusion tag and when isolating captured proteins under native conditions, such as targeting intact cells or cell surface proteins.
Labeling with Hydrazide Reagents
Hydrazides and alkoxyamines are two types of carbonyl-reactive groups. Hydrazides (–NH-NH2) react specifically with aldehyde groups in slightly acidic conditions to form hydrazone linkages; these can be further reduced to stable secondary amine bonds using EDC carbodiimide chemistry.
Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols using . Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4.
Features of EZ-Link Hydrazide-PEG4-Desthiobiotin:
• Desthiobiotin—analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Glycoprotein labeling—tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads
• Cell surface labeling—tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
• Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Pegylated—spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
This desthiobiotin variant of biotin is activated to label periodate-oxidized carbohydrates of macromolecules. The hydrazide group conjugates to carbonyls (aldehydes and ketones), such as those created from sialic acid and other sugar components of glycoprotein polysaccharides upon treatment with 1 to 10 mM sodium periodate (NaIO4). The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative topolyethylene glycol (PEG) spacer arm enhances solubility and reduces aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules. The No-Weigh Format (5 x 1 mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from degradation.
Desthiobiotin vs. Biotin
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (1/Kd = 10^11 vs. 10^15M, respectively)[1-2]. Consequently, desthiobiotinylated bait proteins and their interacting partners can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down assay experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. The modified avidin-biotin affinity system also eliminates the need to use harsh elution conditions that might disassociate complexes and/or damage the target protein or cell. Desthiobiotin-based techniques are ideal when using native or recombinant proteins that are not expressed with a fusion tag and when isolating captured proteins under native conditions, such as targeting intact cells or cell surface proteins.
Labeling with Hydrazide Reagents
Hydrazides and alkoxyamines are two types of carbonyl-reactive groups. Hydrazides (–NH-NH2) react specifically with aldehyde groups in slightly acidic conditions to form hydrazone linkages; these can be further reduced to stable secondary amine bonds using EDC carbodiimide chemistry.
Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols using . Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4.
• Spacer:Extra Long, Pegylated, 31.2 Å
• Labeling Method:Chemical Labeling
• Cell Permeability::Cell-Impermeant
规格
别名
Aladdin™ 酰肼-PEG4-去硫生物素
英文名称
Aladdin™ Hydrazide-PEG4-Desthiobiotin
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
聚合物
技术文档
高级数据
产品属性
| Reactivity | Carboxylic Acid, Ketone, Aldehyde |
|---|
化学和物理性质
溶解性
DMF(Dimethylformamide),DMSO(Dimethylsulfoxide),Water
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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