计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product introduction：
Immobilized Reductant Column contains beaded resin to which an thiol-based reducing agent has been immobilized to enable solid-phase reduction of peptide and protein disulfide bonds.
Features of the Immobilized Reductant Column:
• Eliminates soluble components which interfere with the assay of free thiol groups
• No need to remove reducing agent after the reaction
• Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
• Solid phase; protocols for both peptides and proteins
• Excellent protein recovery
• Regenerable matrix useable up to four times
Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced.
Pierce Immobilized Reductant Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds.Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.
• Form:Liquid
Features of the Immobilized Reductant Column:
• Eliminates soluble components which interfere with the assay of free thiol groups
• No need to remove reducing agent after the reaction
• Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
• Solid phase; protocols for both peptides and proteins
• Excellent protein recovery
• Regenerable matrix useable up to four times
Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced.
Pierce Immobilized Reductant Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds.Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.
• Form:Liquid
规格
别名
Aladdin™ 固定化还原剂柱，2 mL
英文名称
Aladdin™ Immobilized Reductant Column, 2 mL
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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