B2526242计算溶液所需的质量、体积或浓度。
Aladdin® 594 NHS 酯（琥珀酰亚胺酯）
有货
为什么选择此级别
Em:618nm,Ex:590nm 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,干燥,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Aladdin® 594 is a bright red dye. Used for stable signal generation in imaging and flow cytometry, Aladdin® 594 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Aladdin® 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.
The NHS ester (or succinimidyl ester) of Aladdin® 594 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Aladdin® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Detailed information about this Aladdin® NHS ester:
Fluorophore label: Aladdin® 594 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 590/617 nm
Extinction coefficient: 92,000 cm-1M-1
Molecular weight: 819.8
The Aladdin® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.
规格
规格或纯度
Ex:590nm, Em:618nm, mixture of isomers
稳定性与储存
存放在冷冻室（-20 ℃）并避光。
英文名称
Aladdin® 594 NHS Ester (Succinimidyl Ester)
储存条件
避光,-20°C储存,干燥,避免反复冻融
运输条件
超低温运输
Ex/Em(nm)
Ex:590nm;Em:618nm
名称和识别符
技术文档
高级数据
化学和物理性质
溶解性
soluble in water, DMSO, DMF
敏感性
对光敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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