阿拉丁生产的UltraBio™琼脂糖预制胶（TAE），又称琼脂糖预制凝胶或核酸预制胶，是一种安全、便捷、高品质的常用于DNA或RNA样品电泳检测的即用型琼脂糖预制胶。产品质量稳定，使用方便快捷，显著提高实验效率。
产品特点
- 采用自动化灌胶生产技术，确保产品质量的高稳定性和重复性
- 严格控制原料品质，质量稳定
- 方便快捷：放入电泳槽加样通电即可电泳；托盘设计，方便拿取，可直接拍照；特殊配方，23-25V/cm电压，10 min完成电泳
- 安全高效：预加无毒核酸染料，电泳条带敏锐清晰
- 品种齐全：多种浓度可供选择
货号
| 预制胶浓度 | 孔数 | 电泳液
| 凝胶尺寸 | 规格 |
A753104-C1
| 1% | 8 wells | TAE
| 6×6cm | 10片/盒 |
A753104-C2
| 2% | 8 wells
| TAE
| 6×6cm | 10片/盒 |
使用说明
1.将UltraBio™琼脂糖预制胶（TAE）从包装袋中取出，连同托盘一起放入电泳槽中，安装好电泳装置。
2. 上样孔端为负极，然后向槽内加入1×TAE缓冲液至液面恰好没过凝胶表面。如样品孔内有气泡，应除去。
3. DNA样品中加入Loading buffer，混匀，用移液器将样品混合液缓慢加入凝胶加样孔内。上样时需防止将加样孔底部凝胶刺穿。同样的操作方法加入Marker。上样量20-30 μL。
4. 接通电源，红色为正极，黑色为负极。DNA样品由负极往正极泳动（靠近加样孔的一端为负）。电压为120-250V，电泳时间随电泳槽大小变化，电泳槽越大，电泳时间越长。
5. 接通电源，恒压条件下跑胶（150V），当到达实验预定位置时，即可结束电泳。
6. 电泳完毕，关上电源，取出凝胶（带托盘），直接置于凝胶成像系统下观察电泳条带位置并拍照。凝胶内含有的无毒染料，具有与EB相同的光谱特性，可在UV及荧光（594 nm)下观察拍照。
7. 将实验过程中所用电泳设备清洗晾干并置于原位。
注：
1. 本产品预加最新无毒害核酸染料，无需在缓冲液中添加染料。电泳后可直接置于凝胶成像系统下拍照。 染料对单链DNA或RNA的灵敏度低于双链DNA。
2. 成像曝光参数
3. 若需将胶取出，请将刀或任意扁平工具插入凝胶底部，将凝胶从U型托盘中挑起即可。
注意事项
1. 电泳时使用新制的缓冲液可以明显提高电泳效果。注意电泳缓冲液多次使用后，离子强度降低，pH值上升，缓冲性能下降，可能使DNA电泳产生条带模糊和不规则的DNA带迁移的现象。
2. 请勿置于0℃以下，凝胶在0℃以下会冻凝，产生气泡和裂纹，导致凝胶报废。
3. 仅限于专业人员的科学研究用，不得用于临床医学诊断及其他非合理用途。不得存放于普通住宅内。
4. 为了您的安全和健康，请穿戴好个人防护装备和实验服装进行操作。
常见问题及解答
The UltraBio™ Agarose Precast Gel (TAE) produced by Aladdin, also known as an agarose precast gel or nucleic acid precast gel, is a safe, convenient, and high-quality ready-to-use agarose precast gel commonly used for electrophoresis detection of DNA or RNA samples. The product offers stable quality, ease of use, and significantly improves experimental efficiency.
Product Features
Automated gel-casting production technology ensures high stability and reproducibility of product quality.
Strict control of raw material quality ensures consistent performance.
Convenient and fast: Place it in the electrophoresis tank, load samples, and start electrophoresis immediately. Tray design facilitates easy handling and direct imaging. Special formulation allows electrophoresis to be completed in 10 minutes at 23–25 V/cm.
Safe and efficient: Pre-added non-toxic nucleic acid dye provides sharp and clear electrophoresis bands.
Comprehensive range: Available in multiple concentrations.
| Product Code | Gel Concentration | Number of Wells | Electrophoresis Buffer | Gel Dimensions | Specification |
|---|
| A753104-C1 | 1% | 8 wells | TAE | 6×6 cm | 10 gels/box | | A753104-C2 | 2% | 8 wells | TAE | 6×6 cm | 10 gels/box |
|
Instructions for Use
Remove the UltraBio™ Agarose Precast Gel (TAE) from the packaging bag and place it, along with the tray, into the electrophoresis tank. Assemble the electrophoresis setup.
The wells side corresponds to the negative electrode. Add 1× TAE buffer to the tank until the liquid level just covers the gel surface. Remove any bubbles from the wells if present.
Add Loading Buffer to the DNA sample, mix thoroughly, and slowly pipette the sample mixture into the gel wells. Avoid piercing the bottom of the wells during loading. Use the same method to load the Marker. The recommended loading volume is 20–30 μL.
Connect the power supply: red for the positive electrode, black for the negative electrode. DNA samples migrate from the negative to the positive electrode (the end with the wells is negative). Set the voltage to 120–250 V. Electrophoresis time varies with the size of the tank; larger tanks require longer run times.
Run the gel under constant voltage (e.g., 150 V) until the bands reach the desired positions.
After electrophoresis, turn off the power supply, remove the gel (with the tray), and place it directly under a gel imaging system to observe and photograph the bands. The non-toxic dye pre-incorporated in the gel has spectral properties similar to those of EB and can be observed and imaged under UV or fluorescence (594 nm).
Clean, dry, and store the electrophoresis equipment after use.
Notes
This product is pre-loaded with the latest non-toxic nucleic acid dye. No additional dye is required in the buffer. After electrophoresis, the gel can be directly imaged. The dye is less sensitive to single-stranded DNA or RNA compared to double-stranded DNA.
Imaging exposure parameters:
Mode: Reflection
Light source: UV
Exposure time: 1000 ms
To remove the gel from the tray, insert a knife or any flat tool under the gel and lift it from the U-shaped tray.
Precautions
Using freshly prepared buffer for electrophoresis can significantly improve results. Note that repeated use of electrophoresis buffer may reduce ionic strength, increase pH, and decrease buffering capacity, potentially causing blurred bands and irregular DNA migration.
Do not store below 0°C. Gels may freeze, leading to bubbles and cracks, rendering them unusable.
This product is intended for scientific research by professionals only and must not be used for clinical diagnosis or other inappropriate purposes. Do not store in residential areas.
For your safety and health, wear appropriate personal protective equipment and lab attire during operation.
Frequently Asked Questions (FAQs)
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