碱性磷酸酶蓝膜底物溶液已用于：分析直接碱性磷酸酶（AP）活性用比色系统进行碱性磷酸酶测定分析诱导多能干细胞（iPSC）系LND554SV.3的直接碱性磷酸酶（AP）活性在与碱性磷酸酶反应之前，BCIP®试剂为无色至淡蓝色，硝基BT试剂为黄色。在膜型测定中，两组分混合物与碱性磷酸酶形成蓝紫色产物。不建议将该底物用于ELISA分析。应用：

碱性磷酸酶是一种金属酶，属于外切核苷酸酶家族。存在于胆管、骨、肠、胎盘和肿瘤中。

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Application:

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:

to analyse direct alkaline phosphatase (AP) activity
to perform alkaline phosphatase assays with colorimetric system
to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

规格

产品名称

碱性磷酸酶蓝膜底物溶液

规格或纯度

sufficient for 400 mL working substrate

分子类型

生物试剂/缓冲液

储存与运输

物理形态

液体

浓度

sufficient for 400 mL working substrate

储存条件

2-8°C储存

运输条件

低温运输

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

碱性磷酸酶底物检测系统

目录：技术文章

探索主题:

碱性磷酸酶免疫检测试剂和底物检测酶酶抑制剂和酶底物酶促反应检测

Alkaline Phosphatase Substrate Detection System

目录：技术文章

探索主题:

Alkaline phosphatase Immunoassay reagents and substrates Detection enzymes Enzyme inhibitors and enzyme substrates Enzyme-catalyzed reaction detection

碱性磷酸酶（ALP/AKP）活性检测方法比较：酶学特征、底物体系与实验应用选择

目录：技术文章

探索主题:

碱性磷酸酶微量法比色法 ALP活性检测 AKP活性检测 pNPP法磷酸苯二钠法酶活分析

Comparison of Alkaline Phosphatase (ALP/AKP) Activity Assay Methods: Enzymatic Features, Substrate Systems, and Experimental Application Selection

目录：技术文章

探索主题:

Alkaline phosphatase Colorimetric assay ALP activity assays AKP activity assay pNPP method Disodium Phenyl Phosphate Method Microassay Enzyme Activity Analysis

溶液计算器