碱性磷酸酶蓝膜底物溶液已用于:分析直接碱性磷酸酶(AP)活性用比色系统进行碱性磷酸酶测定分析诱导多能干细胞(iPSC)系LND554SV.3的直接碱性磷酸酶(AP)活性在与碱性磷酸酶反应之前,BCIP®试剂为无色至淡蓝色,硝基BT试剂为黄色。在膜型测定中,两组分混合物与碱性磷酸酶形成蓝紫色产物。不建议将该底物用于ELISA分析。应用:
碱性磷酸酶是一种金属酶,属于外切核苷酸酶家族。存在于胆管、骨、肠、胎盘和肿瘤中。
Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.
Application:
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity
- to perform alkaline phosphatase assays with colorimetric system
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3
Prior to reaction with alkaline phosphatase, the BCIP reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.