计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
形态:通过硫酸铵沉淀和DEAE-Sephacel离子交换层析获得的抗血清的IgG组分。
工作稀释:使用ALPH偶联的二抗进行间接ELISA,稀释倍数>1:2000
Western Blot:在1:16000稀释度下,抗血清使用ALPH偶联的二抗与0.75、0.15和0.3 ug过氧化氢酶和1 uL红细胞裂解物反应。
IEP:红细胞裂解物免疫电泳单弧。
用于制备该抗体的过氧化氢酶免疫原在雅典研究与技术实验室纯化。它是一种天然蛋白质,全分子,从人类红细胞中分离出来。
请注意:本产品为实验室试剂,不得用于人体或任何药物用途。仅供研究使用。
Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography.
Working Dilution: >1:2000 by indirect ELISA using ALPH-conjugated secondary antibody
Western Blot: At 1:16000 dilution, antisera reacts to 0.75, 0.15, and 0.3 ug catalase and to 1 uL of red blood cell lysate using ALPH conjugated secondary antibody.
IEP: Single arc upon immunoelectrophoresis of red blood cell lysate.
The Catalase immunogen used to prepare this antibody was purified in the Athens Research & Technology laboratory. It is a native protein, whole molecule, isolated from human erythrocytes.
PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.
Ref:
Kim SJ, Cheresh P, Jablonski RP, Morales-Nebreda L, Cheng Y, Hogan E, Yeldandi A, Chi M, Piseaux R, Ridge K, Michael Hart C, Chandel N, Scott Budinger GR, Kamp DW. Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. Free Radic Biol Med. 2016 Dec;101:482-490. doi: 10.1016/j.freeradbiomed.2016.11.007. Epub 2016 Nov 11.
Xiao W and Goswami PC. Down-regulation of peroxisome proliferator activated receptor γ coactivator 1α induces oxidative stress and toxicity of 1-(4-Chlorophenyl)-benzo-2,5-quinone in HaCaT human keratinocytes. Toxicol In Vitro. 2015 Oct;29(7):1332-8. doi: 10.1016/j.tiv.2015.05.009. Epub 2015 May 22.