Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.
Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.
Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.
The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
宿主种属
兔(Rabbit)
特异性
Amyloid Precursor Protein
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Amyloid beta A4 (AA 700-770).
阳性对照
WB: Mouse brain, Rat brain, HeLa, Hep G2 and HUVEC lysates. IHC: Human brain tissue. IF/ICC: SH-SY5Y cells. IP: HeLa cells.
loid Precursor Protein Antibody (Ab088722) - Western Blot All lanes: Recombinant Amyloid Precursor Protein Antibody (Ab088722) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 99 kDa Exposure time: 38.7 s Amyloid Precursor Protein is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms.
Recombinant Amyloid Precursor Protein Antibody (Ab088722) - Western Blot All lanes use Recombinant Amyloid Precursor Protein Antibody (Ab088722) at 1/1000 dilution for 1 hour at room temperature. Amyloid Precursor Protein is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms.
Recombinant Amyloid Precursor Protein Antibody (Ab088722) - Western Blot All lanes: Recombinant Amyloid Precursor Protein Antibody (Ab088722) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 105, 121, 136 kDa Exposure time: 31.7 s Amyloid Precursor Protein is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms.
Recombinant Amyloid Precursor Protein Antibody (Ab088722) - IP Immunoprecipitate (IP) analysis using Recombinant Amyloid Precursor Protein Antibody (Ab088722) at 1/50 dilution. (WB with Recombinant Amyloid Precursor Protein Antibody (Ab088722) at 1/1000 dilution)
Recombinant Amyloid Precursor Protein Antibody (Ab088722) - IHC Immunohistochemical analysis of paraffin-embedded human brain, using Recombinant Amyloid Precursor Protein Antibody (Ab088722).
Recombinant Amyloid Precursor Protein Antibody (Ab088722) - IF/ICC Immunofluorescent analysis of SH-SY5Y cells, using Recombinant Amyloid Precursor Protein Antibody (Ab088722).
ZJ25F0724200
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