CRY2 Mouse mAb

Name: CRY2 Mouse mAb
SKU: Ab097865
Availability: InStock

货号: Ab097865

COA

有货

级别和纯度: 见COA

应用

Flow Cytometry, IF/ICC, IHC, WB

宿主种属

小鼠(Mouse)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse), 狗(Dog), 猴(Monkey)

亚型

Mouse IgG2a

克隆号

OTI1H5

偶联

Unconjugated

规格

库存

价格

数量

10μl

Ab097865-10μl

现货

¥374.90

50μL

Ab097865-50μl

现货

¥974.90

100μL

Ab097865-100μl

现货

¥1,574.90

1ml

Ab097865-1ml

期货

¥13,999.90

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概述规格储存与运输图片应用技术文档高级数据关联靶点（人）质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Mouse anti Human CRY2 Antibody, Monoclonal (OTI1H5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

规格

产品名称

CRY2 Mouse mAb

别名

CRY2抗体 | CRY2单克隆抗体 | 隐色素2抗体

英文别名

规格或纯度

见COA

生化机理

Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, BMAL1, BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and BMAL1 or BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-BMAL1|BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. Less potent transcriptional repressor in cerebellum and liver than CRY1, though less effective in lengthening the period of the SCN oscillator. Seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY1, dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. May mediate circadian regulation of cAMP signaling and gluconeogenesis by blocking glucagon-mediated increases in intracellular cAMP concentrations and in CREB1 phosphorylation. Besides its role in the maintenance of the circadian clock, is also involved in the regulation of other processes. Plays a key role in glucose and lipid metabolism modulation, in part, through the transcriptional regulation of genes involved in these pathways, such as LEP or ACSL4. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by binding to glucocorticoid response elements (GREs). Represses the CLOCK-BMAL1 induced transcription of BHLHE40/DEC1. Represses the CLOCK-BMAL1 induced transcription of NAMPT. Represses PPARD and its target genes in the skeletal muscle and limits exercise capacity. Represses the transcriptional activity of NR1I2.

宿主种属

小鼠(Mouse)

特异性

CRY2

种属反应性

人(Human),大鼠(Rat),小鼠(Mouse),狗(Dog),猴(Monkey)

免疫原

Recombinant full length protein of human CRY2 produced in HEK293T cell.

阳性对照

WB: Mouse testis lysates. IHC: Human breast adenocarcinoma, colon, colon adenocarcinoma, pancreas carcinoma, thyroid carcinoma, bladder carcinoma, lymphoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY CRY2; HeLa cells. Flow Cytomet

偶联

Unconjugated

克隆类型

单克隆抗体

Format

Whole IgG

纯化方法

Protein A purified

浓度

见COA

分子类型

抗体

产品线

生物产品

储存与运输

物理外观

Liquid

储存缓冲液

10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3.

防腐剂

0.02% Sodium azide

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

短期储存4℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。

图片

Western Blot: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - Western Blot
All lanes: CRY2 Mouse mAb (Ab097865) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 100.0 s

IF/ICC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IF/ICC
Immunofluorescent analysis of COS7 cells transiently transfected by pCMV6-ENTRY CRY2.

IF/ICC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IF/ICC
Immunofluorescent analysis of HeLa cells using CRY2 Mouse mAb (Ab097865).