mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Post-translational: eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
宿主种属
兔(Rabbit)
特异性
eIF5A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human eIF5A1 (AA 1-47).
阳性对照
WB: HeLa, Jurkat, MCF7, Raji, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human uterus cancer tissue. IF/ICC: HeLa cells.
Recombinant eIF5A Antibody (Ab101422) - Western Blot All lanes: Recombinant eIF5A Antibody (Ab101422) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 7.5 s
Recombinant eIF5A Antibody (Ab101422) - IHC Immunohistochemical analysis of paraffin-embedded human uterus cancer, using Recombinant eIF5A Antibody (Ab101422).
Recombinant eIF5A Antibody (Ab101422) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant eIF5A Antibody (Ab101422) at 1/50 dilution.
Recombinant eIF5A Antibody (Ab101422) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant eIF5A Antibody (Ab101422) at 1/50 dilution.
ZJ25F0724970
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