ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti HA tag Antibody, Recombinant, could be used for WB, IF/ICC, Flow Cytometry, IP and so on.
Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 120 kDa
Exposure time: 8.8 s
Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/5000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 2.3 s
Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC
(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with Recombinant HA tag Antibody (Ab106771). The cells were then incubated in the Recombinant HA tag Antibody (Ab106771) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input).
Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input).
1/400 - 1/2000
1/400 - 1/2000
1/10
1/1000 - 1/5000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404375 |
分析证书 | Ab106771 |
| ZJ24F0404376 |
分析证书 | Ab106771 |
| ZJ24F0404377 |
分析证书 | Ab106771 |
| ZJ25F0420132 |
分析证书 | Ab106771 |
| ZJ25F0420133 |
分析证书 | Ab106771 |
| ZJ25F0420131 |
分析证书 | Ab106771 |
| ZJ25F0319481 |
分析证书 | Ab106771 |
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