Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Post-translational: S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
宿主种属
兔(Rabbit)
特异性
HDAC2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human HDAC2 (AA 461-488).
阳性对照
WB: HeLa, HEK293, MCF7, K562 and NIH/3T3 cell lysates. IHC: Human breast tissue. IF/ICC: HeLa cells.
Recombinant HDAC2 Antibody (Ab107032) - Western Blot All lanes: Recombinant HDAC2 Antibody (Ab107032) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 61 kDa Exposure time: 10.5 s
Recombinant HDAC2 Antibody (Ab107032) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HDAC2 Antibody (Ab107032) at 1/50 dilution.
Recombinant HDAC2 Antibody (Ab107032) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HDAC2 Antibody (Ab107032) at 1/50 dilution.
Recombinant HDAC2 Antibody (Ab107032) - IHC Immunohistochemical analysis of paraffin-embedded human breast, using Recombinant HDAC2 Antibody (Ab107032).
Recombinant HDAC2 Antibody (Ab107032) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HDAC2 Antibody (Ab107032) at 1/50 dilution.
ZJ25F0724973
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