May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
宿主种属
兔(Rabbit)
特异性
CDKN1A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human CDKN1A (AA 115-164).
阳性对照
WB: Hep G2, MCF7, HeLa, LNCaP and NIH/3T3 cell lysates. IF/ICC: HeLa, MCF7 cells.
Recombinant p21 Antibody (Ab119613) - Western Blot All lanes: Recombinant p21 Antibody (Ab119613) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa Observed band size: 19 kDa Exposure time: 12.9 s