Epididymis luminal protein 117 antibody | Epididymis secretory protein Li 53 antibody | HEL 117 antibody | HEL S 53 antibody | NDGOA antibody | Neuron cytoplasmic protein 9.5 antibody | OTTHUMP00000218137 antibody | OTTHUMP00000218139 antibody | OTTHUMP00
规格或纯度
无载体,重组,ExactAb™,已验证,PBS Only,无叠氮钠,Lot by Lot
生化机理
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
宿主种属
兔(Rabbit)
特异性
PGP9.5
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属
Cattle,猪(Pig),食蟹猴(Cynomolgus monkey),马(Horse)
免疫原
A synthetic peptide derived from human PGP9.5 (AA 200-223).
阳性对照
WB: Mouse brain, Rat brain, A549, SH-SY5Y and HEK293 lysates. IHC: Rat stomach, Human Hodgkin's lymphoma, ovarian cancer, tonsil, prostate cancer tissues. ICC/IF: HeLa and U87-MG cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
Lot by Lot
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, pH 7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融。
图片
Recombinant PGP9.5 Antibody (Ab121516) - IHC
Immunohistochemical analysis of paraffin-embedded Human tonsil, using Recombinant PGP9.5 Antibody (Ab121516) at 1/200 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IHC
Immunohistochemical analysis of paraffin-embedded Human Hodgkin’s lymphoma, using Recombinant PGP9.5 Antibody (Ab121516) at 1/200 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using Recombinant PGP9.5 Antibody (Ab121516) at 1/200 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IHC
Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant PGP9.5 Antibody (Ab121516) at 1/200 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant PGP9.5 Antibody (Ab121516) at 1/100 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant PGP9.5 Antibody (Ab121516) at 1/25 dilution.
Recombinant PGP9.5 Antibody (Ab121516) - IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using Recombinant PGP9.5 Antibody (Ab121516).
Recombinant PGP9.5 Antibody (Ab121516) - Western Blot
All lanes: Recombinant PGP9.5 Antibody (Ab121516) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 6.2 s
Recombinant PGP9.5 Antibody (Ab121516) - IF/ICC
Immunofluorescent analysis of U87-MG cells, using Recombinant PGP9.5 Antibody (Ab121516).