70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody | Dihydrolipoamide S Acetyltransferase antibody | Dihydrolipoyllysine-residue acetyltransferas
规格或纯度
ExactAb™, 已验证, 敲低验证, 重组, 见COA
生化机理
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
宿主种属
兔(Rabbit)
特异性
Pyruvate Dehydrogenase E2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human DLAT (AA 585-610).
阳性对照
WB: HeLa, Jurkat, Hep G2, MCF7, SH-SY5Y and C6 cell lysates. IF/ICC: HeLa cells.
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) - Western Blot All lanes: Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 63 kDa Exposure time: 48.8 s
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) - Western Blot All lanes use the Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) at 1/1000 dilution for 1 hour at room temperature.
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) at 1/50 dilution.
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124048) at 1/150 dilution.
ZJ25F0723889
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