Renilla luciferin 2 monooxygenase antibody | Renilla type luciferase antibody
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
宿主种属
兔(Rabbit)
特异性
Renilla Luciferase
种属反应性
海肾(Renilla reniformis)
免疫原
A synthetic peptide derived from Renilla Luciferase (AA 1-41).
阳性对照
WB: 293 cells transfected with Renilla Luciferase. IF/ICC: 293 cells transfected with Renilla Luciferase.
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Renilla Luciferase Antibody (Ab125006) - Western Blot All lanes: Recombinant Renilla Luciferase Antibody (Ab125006) at 1/1000 dilution Samples: Lysates at 20 µg per lane
Predicted band size: 36 kDa Observed band size: 35 kDa
Recombinant Renilla Luciferase Antibody (Ab125006) - IF/ICC Immunofluorescent analysis of 293 cells transfected with Renilla Luciferase, using Recombinant Renilla Luciferase Antibody (Ab125006).
ZJ25F0218575
危险品化学品经营许可证（带存储）