ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti V5 tag Antibody, Recombinant, could be used for WB, IF/ICC, Flow Cytometry, IP and so on.
Recombinant V5 tag Antibody (Ab133828) - Western Blot
All lanes: Recombinant V5 tag Antibody (Ab133828) at 1/5000 dilution
Samples: Recombinant V5 protein at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
Exposure time: 0.1 s
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Red) and C-terminal (Green) Recombinant V5 tag Antibody (Ab133828). The cells were then incubated in Recombinant V5 tag Antibody (Ab133828) (1/10000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant V5 tag Antibody (Ab133828) - Western Blot
All lanes: Recombinant V5 tag Antibody (Ab133828) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52, 116 kDa
Observed band size: 52, 116 kDa
Exposure time: 4.3 s
1/50
1/2000 - 1/10000
1/2000 - 1/10000
1/1000-1/3000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0303634 |
分析证书 | Ab133828 |
| ZJ24F0303635 |
分析证书 | Ab133828 |
| ZJ24F0303636 |
分析证书 | Ab133828 |
| ZJ25F0420529 |
分析证书 | Ab133828 |
| ZJ25F0420528 |
分析证书 | Ab133828 |
| ZJ25F0420527 |
分析证书 | Ab133828 |
| ZJ25F0319501 |
分析证书 | Ab133828 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 High Performance 级别指南 →
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