Tight junction protein 1 antibody | Tight junction protein ZO-1 antibody | Tight junction protein ZO1 antibody | TJP1 antibody | zo-1 antibody | Zo1 antibody | ZO1_HUMAN antibody | Zona occludens 1 antibody | Zona occludens 1 protein antibody | Zona occlu
规格或纯度
无载体, ExactAb™, 已验证, 见COA
生化机理
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions.
宿主种属
兔(Rabbit)
特异性
ZO1 tight junction
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant full length protein of human ZO1 tight junction.
阳性对照
WB: C2C12 and HEK293 cell lysates. IF/ICC: MCF7 cells.
ZO1 tight junction Antibody (Ab135462) - Western Blot All lanes: ZO1 tight junction Antibody (Ab135462) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 195 kDa Observed band size: 250 kDa Exposure time: 34.2 s
ZO1 tight junction Antibody (Ab135462) - Western Blot All lanes: ZO1 tight junction Antibody (Ab135462) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 195 kDa Observed band size: 245 kDa Exposure time: 29.6 s
ZO1 tight junction Antibody (Ab135462) - IF/ICC IF analysis of ZO1 tight junction Antibody (Ab135462) in MCF7 cells. The cells were 4% paraformaldehyde-fixed, 0.3% Triton X-100 for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ZO1 tight junction Antibody (Ab135462) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab223319) at 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ25F0724329
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