Purified goat polyclonal antibody, conjugated with Alkaline Phosphatase, suitable for WB and ELISA.
规格
产品名称
Goat Anti-Human IgG (Alkaline Phosphatase)
别名
羊抗人IgG抗体 | 羊抗人IgG | 山羊抗人IgG | 山羊抗人IgG抗体
英文别名
Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I
规格或纯度
ExactAb™, 已验证, 见 COA
宿主种属
山羊(Goat)
种属反应性
人(Human)
免疫原
human IgG whole molecule
偶联
Alkaline Phosphatase
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
antigen affinity chromatography
浓度
See COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in 50mM tris with 1mM Magnesium chloride, 0.15M NaCl, pH 8.0, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) - ELISA Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) was tested by direct ELISA. The plate was coated with 1 µg/mL (100 µL/well) Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature. .
Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) - Western Blot All lanes: Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) at 1/1000 dilution Samples: Lysates at 2.0 µg per lane Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature. Predicted band size: 50 kDa Observed band size: 27, 53 kDa