Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
宿主种属
小鼠(Mouse)
特异性
PCNA
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属
兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken)
免疫原
Full length recombinant human PCNA
阳性对照
IF/ICC: MCF-7 cells Flow Cytometry: Hela cells
偶联
FITC
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
PCNA Mouse mAb (FITC) (Ab175853) - ICC/IF IF analysis of PCNA (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (FITC) (Ab175853) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (FITC) (Ab175853) - Flow Cytometry Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (FITC) (Ab175853) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (FITC) (Ab180307).
ZJ25F0825432
危险品化学品经营许可证（带存储）