Liquid in 50mM tris with 1mM Magnesium chloride, 0.15M NaCl, pH 8.0, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - Western Blot All lanes: beta Actin Antibody (Ab090999) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) at 1/20000 dilution Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 0.6 s
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - ELISA Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature.