Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
宿主种属
亚美尼亚仓鼠(Armenian hamster)
特异性
CD80
种属反应性
小鼠(Mouse)
免疫原
CHO cell line transfected with mouse B7 (CD80).
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
Format
Whole IgG
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
CD80 Armenian Hamster mAb (PE) (Ab179739) - Flow Cytometry
LPS (L118716) stimulated (2.5 days) C57BL/6 Mouse splenocytes were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with CD80 Armenian Hamster mAb (PE) (Ab179739) (red) at 0.1μg/test for 1 hour at 4°C compared to an isotype control (blue). Blue - Armenian Hamster IgG (PE) (Ab191748). Black - Unlabelled control, cells without incubation with primary antibody. Unstimulated cells were used as control (left panel).
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.