Application: Flow: ≤1.0 μg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
图片
CD127 Rat mAb (Biotin) (Ab185388) - Flow Cytometry Flow cytometry analysis of CD127 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD127 Rat mAb (Biotin) (Ab185388) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).
CD127 Rat mAb (Biotin) (Ab185388) - Flow Cytometry Flow cytometry analysis of CD127 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD127 Rat mAb (Biotin) (Ab185388) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.