Rat IgG; Polyclonal, Isotype Control, could be used for Flow Cytometry.
规格
产品名称
Rat IgG (AF594)
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
大鼠(Rat)
偶联
AF594
Excitation(nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Affinity chromatography
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Rat IgG (AF594) (Ab190241) - Flow Cytometry Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF594) (Ab191793) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF594) (Ab190241).
Rat IgG (AF594) (Ab190241) - Flow Cytometry Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were preincubated Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF594) (Ab191793) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF594) (Ab190241). Black - Unlabelled control, cells without incubation with primary antibody.
Rat IgG (AF594) (Ab190241) - Flow Cytometry Two-color flow cytometric analysis of CD200/OX2 expression on mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF594) (Ab190241; Left panel) or CD200/OX2 Rat mAb (AF594) (Ab190186; Right panel) at 0.3μg/test.
应用
应用名称
稀释比例
Flow Cytometry
Use at an assay dependent concentration. Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci