Rat anti Mouse GITR/CD357 Antibody, Monoclonal (DTA-1), could be used for Flow Cytometry.
规格
产品名称
GITR/CD357 Rat mAb (PE-Cy7)
别名
糖皮质激素诱导肿瘤坏死因子受体抗体 | GITR/CD357抗体
英文别名
Gitr | Activation-inducible TNFR family receptor | AITR | AITRTNF receptor superfamily activation-inducible protein | CD357 antigen | CD357 | GITR-D | GITRtumor necrosis factor receptor superfamily member 18 | Glucocorticoid-induced TNFR-related protein |
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
宿主种属
大鼠(Rat)
特异性
GITR/CD357
种属反应性
小鼠(Mouse)
免疫原
Mouse CD25+ CD4+ T cells
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes.
偶联
PE-Cy7
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
774nm
Ex/Em(nm)
Ex:565nm;Em:774nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
GITR/CD357 Rat mAb (PE-Cy7) (Ab190292) - Flow Cytometry Two-color flow cytometric analysis of GITR/CD357 expression on mouse splenocytes. Cells were preincubated with Mouse Fc Receptor Blocking Solution Plus (M1374079). The cells were then stained with CD25 Rat mAb (AF647) (Ab211659) and either Rat IgG (PE-Cy7) (Ab190073; Left panel) or GITR/CD357 Rat mAb (PE-Cy7) (Ab190292; Right panel) at 0.3 μg/test. Total viable cells were used for analysis.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F0926946
危险品化学品经营许可证（带存储）