Rat anti Mouse SIGLEC H Antibody, Monoclonal (440c), could be used for Flow Cytometry.
规格
产品名称
SIGLEC H Rat mAb (FITC)
别名
Siglec-H抗体
英文别名
sialic acid binding Ig like lectin H antibody | SIGLEC like protein antibody | Siglech antibody | Siglec-H | 6430529G09Rik
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
大鼠(Rat)
特异性
SIGLEC H
种属反应性
小鼠(Mouse)
免疫原
Purified bone marrow-derived IPCs
阳性对照
Flow Cytometry: BALB/C Mouse splenocytes
偶联
FITC
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
SIGLEC H Rat mAb (FITC) (Ab191591) - Flow Cytometry Multicolor flow cytometric analysis of SIGLEC H expression on mouse leucocytes. BALB/c mouse splenocytes were preincubated with CD16/CD32 Rat mAb (A412018). Cells were stained with CD45R Rat mAb (PE-Cy7) (Ab191673), CD11c Armenian Hamster mAb (APC) (Ab178872) and either Rat IgG (FITC) (Ab180311; Left panel) or SIGLEC H Rat mAb (FITC) (Ab191591; Right panel) at 0.1 μg/test. A bivariate pseudocolor density plot showing the correlated expression of SIGLEC H expression (or Isotype control staining) was derived from CD45R+ gated events with the forward and side light-scatter characteristics of viable thymocytes.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.