Rat anti Mouse SIRP alpha/CD172a Antibody, Monoclonal (P84), could be used for Flow Cytometry.
规格
产品名称
SIRP alpha/CD172a Rat mAb (AF488)
别名
信号调节蛋白α(SIRPa)抗体 | SIRP alpha/CD172a抗体
英文别名
Bit | SIRP | SHP-1 | Ptpns1 | Idd13.2 | BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs | Brain Ig-like molecule with tyrosine-based activation motifs | CD172 antigen-like family member A | CD172a antigen | CD172a | Inhibitory
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
宿主种属
大鼠(Rat)
特异性
SIRP alpha/CD172a
种属反应性
小鼠(Mouse)
免疫原
Mouse brain membrane protein
阳性对照
Flow: C57BL/6 mouse bone marrow cells.
偶联
AF488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) - Flow Cytometry Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).
SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) - Flow Cytometry Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F0825214
危险品化学品经营许可证（带存储）