Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
宿主种属
大鼠(Rat)
特异性
c-Kit/CD117
种属反应性
小鼠(Mouse)
免疫原
IL-3-dependent mast cells
阳性对照
Flow Cytometry: C57BL/6 mouse bone marrow cells
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
Format
Whole IgG
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
c-Kit/CD117 Rat mAb (PE) (Ab209974) - Flow Cytometry Two-color flow cytometric analysis of c-Kit/CD117 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (PE) (Ab180312; Left panel) or c-Kit/CD117 Rat mAb (PE) (Ab209974; Right panel) at 0.3μg/test.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
ZJ25F0724044
危险品化学品经营许可证（带存储）