at anti Mouse NKG2D/CD314 Antibody, Monoclonal (CX5), could be used for Flow Cytometry.
规格
产品名称
NKG2D/CD314 Rat mAb (Biotin)
别名
杀伤细胞凝集素样受体亚家族K成员1(KLRK1)抗体
英文别名
Nkg2d | D6H12S2489E | CD314 antigen | CD314 | D12S2489E | FLJ17759 | FLJ75772 | Killer cell lectin-like receptor subfamily K member 1 | killer cell lectin-like receptor subfamily K, member 1 | KLR | KLRK1 | NK cell receptor D | NKG2-D type II integral mem
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
宿主种属
大鼠(Rat)
特异性
NKG2D/CD314
种属反应性
小鼠(Mouse)
免疫原
Purified mouse NKG2D
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Biotin
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
NKG2D/CD314 Rat mAb (Biotin) (Ab211175) - Flow Cytometry Two-color flow cytometric analysis of NKG2D/CD314 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with NKG2A/C/E Rat mAb (AF647) (Ab212165) and either Rat IgG (Biotin) (Ab180310; Left panel) or NKG2D/CD314 Rat mAb (Biotin) (Ab211175; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.