May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
宿主种属
大鼠(Rat)
特异性
CD90.2/Thy1.2
种属反应性
小鼠(Mouse)
免疫原
Mouse thymus or spleen
阳性对照
Flow: C57BL/6 mouse splenocytes
偶联
Biotin
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122) - Flow Cytometry Two-color flow cytometric analysis of CD90.2/Thy1.2 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122; Right panel) at 0.1μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.
Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122) - Flow Cytometry Flow cytometry analysis of CD90.2/Thy1.2 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).
Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122) - Flow Cytometry Flow cytometry analysis of CD90.2/Thy1.2 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD90.2/Thy1.2 Antibody (Biotin) (Ab213122) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.