This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. Ready-to-use secondary antibody concentrations have been optimized and tested with ELISA and WB. No calculations and dilutions are required. Just add an assay dependent volume to your specimen, and you're ready for the next step in your experiment.
Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I
规格或纯度
ExactAb™, 已验证, 见COA
宿主种属
山羊(Goat)
种属反应性
小鼠(Mouse)
交叉反应
Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原
Mouse IgG
偶联
Biotin
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
antigen affinity chromatography
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze.
图片
Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Recommend adding 10mL of Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) solution for each mini gel. Note that the membrane needs to be totally immersed in solution. We used 4mL of ready-to-use reagent to incubate the PVDF membrane (8cm x 4cm) in this experiment. SA-HRP: Streptavidin (HRP) (np156148) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 40 kDa Exposure time: 100.0 s
Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) - ELISA Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) was tested by ELISA. The plate was coated with Mouse IgG (Ab170221) at a series of different dilution ratios. Goat Anti-Mouse IgG H&L (Biotin) (Ready to use) (Ab218687) was added at 100 μL/well. Then Streptavidin-HRP (np156148) was used at 1/20000 dilution for this ELISA.
ZJ25F0623249
危险品化学品经营许可证（带存储）