Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from mouse. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I
规格或纯度
ExactAb™, 已验证, 见 COA
宿主种属
山羊(Goat)
特异性
Mouse IgG
种属反应性
小鼠(Mouse)
交叉反应
Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原
Mouse IgG
偶联
Cy3
Excitation(nm)
Green Laser(532nm)
Emission(nm)
566nm
Ex/Em(nm)
Ex:554nm;Em:566nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
antigen affinity chromatography
浓度
See COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
图片
Goat Anti-Mouse IgG H&L (Cy3) (Ready to use) (Ab218691) - IF/ICC IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 1 μg/mL Catumaxomab (anti-CD3&EpCAM) (Ab175489) in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Mouse IgG H&L (Cy3) (Ready to use) (Ab218691) at 1mL/well for 1 hour at room temperature in the dark (red). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (Cy3) (Ready to use) (Ab218691) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (Cy3) (Ready to use) (Ab218691) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0826306
危险品化学品经营许可证（带存储）