This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. Ready-to-use secondary antibody concentrations have been optimized and tested with ELISA and WB. No calculations and dilutions are required. Just add an assay dependent volume to your specimen, and you're ready for the next step in your experiment.
宿主种属
山羊(Goat)
特异性
Rabbit IgG
种属反应性
兔(Rabbit)
免疫原
Rabbit IgG
偶联
Biotin
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Antigen affinity purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
图片
Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Recommend adding 10mL of Goat Anti-Rabbit IgG H&L (Biotin) (Ab218693) solution for each mini gel. Note that the membrane needs to be totally immersed in solution. We used 4mL of ready-to-use reagent to incubate the PVDF membrane (8cm x 4cm) in this experiment. SA-HRP: Streptavidin (HRP) (np156148) at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 200.0 s
Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use) (Ab218693) - ELISA Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use) (Ab218693) was tested by ELISA. The plate was coated with Rabbit IgG (Ab170215) at a series of different dilution ratios. Goat Anti-Rabbit IgG H&L (Biotin) (Ready to use) (Ab218693) was added at 100 μL/well. Then Streptavidin-HRP (np156148) was used at 1/20000 dilution for this ELISA.
ZJ25F0826286
危险品化学品经营许可证（带存储）