Recombinant full length protein corresponding to Human Hsp60
阳性对照
IF/ICC: MCF-7 cells
偶联
AF750
Excitation(nm)
Red Laser(633nm)
Emission(nm)
776nm
Ex/Em(nm)
Ex:752nm;Em:776nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
Hsp60 Mouse mAb (AF750) (Ab219139) – IF/ICC IF analysis of Hsp60 (red) in MCF-7 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with Hsp60 Mouse mAb (AF750) (Ab219139) at a dilution of 1/1000 in blocking buffer for 2 hour at RT. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.