WB: HeLa, MCF-7, HepG2, NIH3T3 and C6 whole cell lysates Flow Cytometry: Hela cells IF/ICC: Hela cells
偶联
Biotin
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
alpha Tubulin Mouse mAb (Biotin) (Ab219159) – IF/ICC IF analysis of alpha Tubulin (red) in Hela cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Western Blot All lanes: alpha Tubulin Mouse mAb (Biotin) (Ab219159) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: SA-HRP (np156148) at 1/10000 dilution
Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 120.0 s
alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Flow Cytometry Flow cytometry analysis of alpha Tubulin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) (red) at 1/50 dilution for 30 minutes at 4°C in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0826373
危险品化学品经营许可证（带存储）