Recombinant protein corresponding to Human Lamin A
阳性对照
Flow Cytometry: HeLa cells ICC/IF: HeLa cells
偶联
AF647
Excitation(nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
Lamin A/C Mouse mAb (AF647) (Ab219218) - IF/ICC IF analysis of Lamin A/C (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (AF647) (Ab219218) at a dilution of 1/1000 in blocking buffer for 2 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Lamin A/C Mouse mAb (AF647) (Ab219218) - Flow Cytometry Flow cytometry analysis of Lamin A/C expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Lamin A/C Mouse mAb (AF647) (Ab219218) (Red) at a dilution of 1/900 for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.