Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from human. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
规格
产品名称
Goat Anti-Human IgG (AF405) (Ready to use)
别名
羊抗人IgG抗体 | 羊抗人IgG | 山羊抗人IgG | 山羊抗人IgG抗体
英文别名
Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I
规格或纯度
ExactAb™, 已验证, 见 COA
宿主种属
山羊(Goat)
特异性
Human IgG
种属反应性
人(Human)
偶联
AF405
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
antigen affinity chromatography
浓度
See COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
图片
Goat Anti-Human IgG (AF405) (Ready to use) (Ab220096) - IF/ICC IF analysis of EpCAM (blue) in HT-29 cells. The cells were fixed with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Human IgG (AF405) (Ready to use) (Ab220096) at 1mL/well for 1 hour at room temperature in the dark (blue). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.
Goat Anti-Human IgG (AF405) (Ready to use) (Ab220096) - Flow Cytometry Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (AF405) (Ready to use) (Ab220096) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F1028520
危险品化学品经营许可证（带存储）