Purified goat polyclonal antibody, suitable for Flow Cytometry and IF/ICC.
规格
产品名称
Goat Anti-Human IgG (LumiDye™ Yellow-Green 568)
别名
羊抗人IgG抗体 | 羊抗人IgG | 山羊抗人IgG | 山羊抗人IgG抗体
英文别名
Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I
规格或纯度
ExactAb™, 已验证, 1.0 mg/mL
宿主种属
山羊(Goat)
种属反应性
人(Human)
偶联
LumiDye™ Yellow-Green 568
Excitation(nm)
Green Laser(532nm)
Emission(nm)
568nm
Ex/Em(nm)
Ex:553nm;Em:568nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
antigen affinity chromatography
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Human IgG (LumiDye™ Yellow-Green 568) (Ab220542) -IF/ICC IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (LumiDye™ Yellow-Green 568) (Ab220542) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Human IgG (LumiDye™ Yellow-Green 568) (Ab220542) - Flow Cytometry Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (LumiDye™ Yellow-Green 568) (Ab220542) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0623174
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