Mouse anti HA tag Antibody, Monoclonal antibody(12CA5), could be used for Flow Cytometry, IF/ICC and so on.
规格
产品名称
HA tag Mouse mAb (FITC)
别名
HA tag 标签单克隆抗体 | HA tag 单克隆抗体 | HA tag 抗体
英文别名
HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody
规格或纯度
高性能, 已验证, ExactAb™, 0.5 mg/mL
生化机理
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
宿主种属
小鼠(Mouse)
特异性
HA
种属反应性
不依赖种属(Species independent)
免疫原
Influenza virus hemagglutinin protein (AA 98-106).
阳性对照
Flow Cytometry: HEK293 cells transfected with HA and mCherry tags IF/ICC: HEK293 cells transfected with HA and mCherry tags
偶联
FITC
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
克隆类型
单克隆抗体
Format
Whole IgG
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
HA tag Mouse mAb (FITC) (Ab222049) - IF/ICC IF analysis of HA tag (Green) in HEK293T cells transfected with a mCherry-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HA tag Mouse mAb (FITC) (Ab222049) at 1/1000 dilution in blocking buffer for 2 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
HA tag Mouse mAb (FITC) (Ab222049) - Flow Cytometry Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with a mCherry-HA tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with HA tag Mouse mAb (FITC) (Ab222049) at a dilution of 1/500 for 30 minutes at 4°C in the dark. Blue - Mouse IgG (FITC) (Ab180307).
ZJ25F0926911
危险品化学品经营许可证（带存储）