Mouse anti His tag (C-terminal) Antibody, clone IJ08/4E6, could be used for WB and IF/ICC.
规格
产品名称
His tag (C-terminal) Mouse mAb (Biotin)
别名
聚组氨酸标签单克隆抗体 | 聚组氨酸抗体 | His tag标签抗体 | His tag单克隆抗体 | 6xHis抗体 | His标签 | His标签抗体 | His标签小鼠单克隆抗体 | His抗体
英文别名
6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | His tag Mouse Monoclonal Antibody | His tag Mouse mAb | Anti-His tag | Monoclonal Anti- His tag antibo
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
宿主种属
小鼠(Mouse)
特异性
Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
种属反应性
不依赖种属(Species independent)
免疫原
A recombinant protein containing the sequence HHHHHH
阳性对照
WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins
偶联
Biotin
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) - Western Blot All lanes: His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: SA-HRP (np156148) at 1/10000 dilution
Predicted band size: 113 kDa Observed band size: 52, 113 kDa Exposure time: 120.0 s
ZJ25F0724167
危险品化学品经营许可证（带存储）