Flow Cytometry: Human peripheral blood lymphocytes
偶联
PE-Cy5
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
670nm
Ex/Em(nm)
Ex:565nm;Em:670nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
Site-Specific Conjugation;5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD3 Antibody (PE-Cy5) (Ab222072) - Flow Cytometry Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (PE-Cy5) (Ab222072) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5) (Ab190078).
Recombinant CD3 Antibody (PE-Cy5) (Ab222072) - Flow Cytometry Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (PE-Cy5) (Ab222072) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.