No cross reaction was detected with human IgG, mouse IgG, horse IGG, pig IgG and chicken IGY.
免疫原
Rabbit IgG
偶联
AF546
Excitation(nm)
Green Laser(532nm)
Emission(nm)
570nm
Ex/Em(nm)
Ex:554nm;Em:570nm
克隆类型
多克隆抗体
Format
Whole IgG
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Rabbit IgG H&L (AF546) (Ab223314) - IF/ICC IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF546) (Ab223314) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.