Coreceptor for bacterial lipopolysaccharide.
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS).
Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway.
Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL.
宿主种属
小鼠(Mouse)
特异性
Human CD14
种属反应性
人(Human)
免疫原
Human Thymocytes followed by peripheral blood mononuclear cells.
阳性对照
Flow Cytometry: Human peripheral blood monocytes
偶联
PE-AF647
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:565nm;Em:668nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD14 Antibody (PE-AF647) (Ab223480) - Flow Cytometry Flow cytometry analysis of CD14 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD14 Antibody (PE-AF647) (Ab223480) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-AF647) (Ab216766).
Recombinant CD14 Antibody (PE-AF647) (Ab223480) - Flow Cytometry Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD14 Antibody (PE-AF647) (Ab223480) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-AF647) (Ab216766). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ25F0825115
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