Recombinant CD16 Antibody, clone P06F04, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD16 Antibody (Biotin)
别名
FcγRIII抗体 | CD16 抗体 | Anti-CD16抗体
英文别名
CD 16 antibody | CD 16a antibody | CD16 antibody | CD16a antibody | CD16a antigen antibody | CD16B antibody | CD16b antigen antibody | Fc fragment of IgG antibody | Fc fragment of IgG low affinity IIIa receptor (CD16) antibody | Fc fragment of IgG low aff
规格或纯度
重组, ExactAb™, 已验证, 0.5mg/mL
生化机理
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
宿主种属
小鼠(Mouse)
特异性
CD16
种属反应性
人(Human)
免疫原
Human NK Cells
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
Biotin
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD16 Antibody (Biotin) (Ab223488) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD16 Antibody (Biotin) (Ab223488) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).
Recombinant CD16 Antibody (Biotin) (Ab223488) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD16 Antibody (Biotin) (Ab223488) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F0928061
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