FLJ51114 | histocompatibility antigen HLA-DR alpha | HLA class II histocompatibility antigen | DR alpha chain | HLADR | HLA-DR | HLA-DRA | HLA-DRA1 | major histocompatibility complex | class II | DR alpha | MHC cell surface glycoprotein | MHC class II ant
规格或纯度
重组, ExactAb™, 已验证, 5 μL/test
生化机理
An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells.
Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes.
In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins.
Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance.
The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides.
The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
宿主种属
小鼠(Mouse)
特异性
Human HLA-DR
种属反应性
人(Human)
免疫原
Human lymphoblastoid B-cell line RPMI 8866
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
偶联
AF647
Excitation(nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant HLA-DR Antibody (AF647) (Ab223635) - Flow Cytometry Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant HLA-DR Antibody (AF647) (Ab223635) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF647) (Ab190077).
Recombinant HLA-DR Antibody (AF647) (Ab223635) - Flow Cytometry Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF647) (Ab223635) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant HLA-DR Antibody (AF647) (Ab223635) - Flow Cytometry Flow cytometry analysis of HLA-DR expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF647) (Ab223635) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ25F0826064
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