B220 antibody | CD45 antibody | CD45 antigen antibody | Glycoprotein antibody | GP180 antibody | L CA antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | Leukocyte common antigen precursor antibody | loc antibody | Ly-5 antibody
规格或纯度
重组, ExactAb™, 已验证, 0.5 mg/mL
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity).
Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45R Antibody (AF405) (Ab223839) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45R Antibody (AF405) (Ab223839) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0130972
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