Recombinant CD45R Antibody, clone P42D24, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD45R Antibody (PE-Cy5)
别名
蛋白酪氨酸磷酸酶受体C(CD45)抗体 | CD45R抗体
英文别名
B220 antibody | CD45 antibody | CD45 antigen antibody | Glycoprotein antibody | GP180 antibody | L CA antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | Leukocyte common antigen precursor antibody | loc antibody | Ly-
规格或纯度
ExactAb™,已验证,重组,0.5 mg/mL
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity).
Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45R Antibody (PE-Cy5) (Ab223853) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD45R Antibody (PE-Cy5) (Ab223853) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ26F0131460
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