Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells.
Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
Post-translational modification
Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome. Ubiquitinated by RNF128 via 'Lys-27'-linked polyubiquitination, facilitating its degradation through the lysosomal pathway.
宿主种属
小鼠(Mouse)
特异性
Human Interleukin-3 receptor subunit alpha
种属反应性
人(Human)
免疫原
Human IL-3Rα transfected COS cells.
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
Pacific Blue
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant IL3RA/CD123 Antibody (Pacific Blue) (Ab223898) - Flow Cytometry Flow cytometry analysis of IL3RA/CD123 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant IL3RA/CD123 Antibody (Pacific Blue) (Ab223898) at 1 μL/test for 1 hour at 4°C. Left panel - Mouse IgG (Pacific Blue) (Ab216764).
Recombinant IL3RA/CD123 Antibody (Pacific Blue) (Ab223898) - Flow Cytometry Flow cytometry analysis of IL3RA/CD123 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant IL3RA/CD123 Antibody (Pacific Blue) (Ab223898) at 1 μL/test for 1 hour at 4°C. Blue - Mouse IgG (Pacific Blue) (Ab216764). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
1μl/Test
For flow cytometric staining, the suggested use of this reagent is 1 µL per million cells in 100 µL staining volume or 1 µL per 100 µL of whole blood.
ZJ25F1028720
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