B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes.
Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
宿主种属
小鼠(Mouse)
特异性
Human CD20
种属反应性
人(Human)
免疫原
Human 6.16c1.3 B cell line
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
Pacific Blue
Excitation(nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD20 Antibody (Pacific Blue) (Ab223966) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD20 Antibody (Pacific Blue) (Ab223966) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (Pacific Blue) (Ab216764).
Recombinant CD20 Antibody (Pacific Blue) (Ab223966) - Flow Cytometry Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD20 Antibody (Pacific Blue) (Ab223966) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (Pacific Blue) (Ab216764). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
5 μL/test;
For flow cytometric staining, the suggested use of this reagent is 5 µL per 100,000 - million cells in a 100 µL volume of Annexin V Binding Buffer. It
ZJ25F0928160
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